Genome-Wide CRISPR Screen Identifies Phospholipid Scramblase 3 as the Biological Target of Mitoprotective Drug SS-31.

Josie A SilvaroliBijay BisunkeJi Young KimAmanda Stayton Laura A Jayne Shirely A Martinez Christopher NguyenPrisha S PatelThitinee Vanichapol Vivek Verma Juheb Akhter Subhashini Bolisetty Sethu M Madhavan Cem Kuscu Christopher C Coss Diana Zepeda-OrozcoSamir V ParikhAnjali A Satoskar Alan James Davidson James D Eason Hazel H Szeto Navjot Singh Pabla Amandeep Bajwa

Published in: Journal of the American Society of Nephrology : JASN (2024)

PLSCR3 is identified as the essential biological target that facilitates the mitoprotective effects of SS-31 in vitro and in vivo. PLSCR3 agonists can alleviate mitochondrial dysfunction linked to AKI.

Keyphrases

genome wide
dna methylation
copy number
acute kidney injury
high throughput
gene expression
crispr cas
fatty acid
genome editing
emergency department