Keth-seq for transcriptome-wide RNA structure mapping.

Xiaocheng WengJing GongYi ChenTong WuFang WangShixi YangYushu YuanGuan-Zheng LuoKai ChenLulu HuHonghui MaPingluan WangQiangfeng Cliff ZhangXiang ZhouChuan He

Published in: Nature chemical biology (2020)

RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.

Keyphrases

nucleic acid
rna seq
single cell
genome wide
high resolution
gene expression
induced apoptosis
high density
cell proliferation
cell death