The aim of the present study was to investigate the effects of substituting dietary barley grain by different sources and levels of fat on performance and meat quality of Holstein young bulls. Twenty-eight Holstein male calves, 274 ± 32 kg initial body weight, were used in a completely randomised design with a 2 × 2 factorial treatment arrangement for 100 d (with 10 d of adaptation). Seven animals were randomly allocated per treatment. Four dietary treatments were tested, which included: (1) diet supplemented with saturated fat (Energizer® RP10) at low level (diet with high barley and starch content), (2) diet supplemented with saturated fat at high level (diet with low barley and starch content), (3) diet supplemented with unsaturated fat (Persiafat) at low level (diet with high barley and starch content) and (4) diet supplemented with unsaturated fat at high level (diet with low barley and starch content). The dry matter intake, average daily gain, feed conversion ratio and carcass traits were not affected by supplemental fat sources and levels. Total tract digestibility of acid detergent fibre was improved after inclusion of both rumen-protected fat sources while other nutrient digestibilities were not affected by the fat sources or levels. In conclusion, partial replacing of dietary starch with fat supplements has not negative effects on calves' performance and digestibility of neutral detergent fibre.