Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins.
Alba Gutiérrez-DocioPaula AlmodóvarSilvia Moreno-FernandezJose Manuel SilvanAdolfo J Martinez-RodriguezGonzalo Luis AlonsoMarin ProdanovPublished in: Membranes (2020)
The effectiveness of a preparative integrated ultrafiltration/solid-phase extraction (UF/SPE) process for purification of oligomeric procyanidins (OPCs) from a crude grape seed extract (GSE) was studied for the first time. The separation of OPCs from polymeric procyanidins (PPCs) by UF was very efficient. The membrane showed an acceptable filtration flux of 6 to 3.5 L/h·m2 at 0.5 bar of transmembrane pressure and 95% recovery of its water flux after chemical cleaning. The process was scalable to a pilot scale. The separation of very polar and ionic species from OPCs by SPE (XAD7HP and XAD16 resins) was also very good, but both adsorbents lost their retention capacities quickly, due probably to irreversible retention of OPCs/PPCs. Even though the global purification of OPCs by the integrated UF/SPE process allowed the recovery of 24.2 g of highly purified OPCs (83% purity) from 14.4 L of crude grape seed extract, the use of these adsorbents for further purification of the OPCs was very limited.
Keyphrases
- solid phase extraction
- liquid chromatography
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- tandem mass spectrometry
- gas chromatography mass spectrometry
- simultaneous determination
- ultra high performance liquid chromatography
- ms ms
- mass spectrometry
- gas chromatography
- high resolution mass spectrometry
- randomized controlled trial
- oxidative stress
- drug delivery
- systematic review
- recombinant human
- ionic liquid
- high resolution
- study protocol
- drug release
- double blind