Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus.
Daria ProkhorovaNatalya Zhukova EschenkoAnna LemzaMariia V SergeevaRinat AmirkhanovGrigory A StepanovPublished in: Viruses (2022)
Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
Keyphrases
- genome editing
- crispr cas
- acute respiratory distress syndrome
- genome wide
- immune response
- life cycle
- extracorporeal membrane oxygenation
- sars cov
- bioinformatics analysis
- induced apoptosis
- genome wide identification
- endothelial cells
- mechanical ventilation
- poor prognosis
- single cell
- genome wide analysis
- cell therapy
- stem cells
- dna methylation
- dendritic cells
- signaling pathway
- oxidative stress
- inflammatory response
- mesenchymal stem cells
- endoplasmic reticulum stress