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Discovery of widespread type I and type V CRISPR-Cas inhibitors.

Nicole D MarinoJenny Y ZhangAdair L BorgesAlexander A SousaLina M LeonBenjamin J RauchRussell T WaltonJoel D BerryJ Keith JoungBenjamin P KleinstiverJoseph Bondy-Denomy
Published in: Science (New York, N.Y.) (2018)
Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here, we report the discovery of 12 acr genes, including inhibitors of type V-A and I-C CRISPR systems. AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs-including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a-when assayed in human cells. The acr genes reported here provide useful biotechnological tools and mark the discovery of acr loci in many bacteria and phages.
Keyphrases
  • crispr cas
  • genome editing
  • genome wide
  • small molecule
  • high throughput
  • copy number
  • genome wide identification
  • bioinformatics analysis
  • transcription factor
  • gene expression
  • quantum dots
  • single molecule