Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro .
Ewa Bielczyk-MaczynskaPublished in: Adipocyte (2024)
Dedifferentiated adipose tissue (DFAT) has been proposed as a promising source of patient-specific multipotent progenitor cells (MPPs). During induced dedifferentiation, adipocytes exhibit profound gene expression and cell morphology changes. However, dedifferentiation of post-mitotic cells is expected to enable proliferation, which is critical if enough MPPs are to be obtained. Here, lineage tracing was employed to quantify cell proliferation in mouse adipocytes subjected to a dedifferentiation-inducing protocol commonly used to obtain DFAT cells. No evidence of cell proliferation in adipocyte-derived cells was observed, in contrast to the robust proliferation of non-adipocyte cells present in adipose tissue. We conclude that proliferative MPPs derived using the ceiling culture method most likely arise from non-adipocyte cells in adipose tissue.
Keyphrases
- adipose tissue
- induced apoptosis
- cell cycle
- cell proliferation
- insulin resistance
- cell cycle arrest
- gene expression
- high fat diet
- signaling pathway
- randomized controlled trial
- endoplasmic reticulum stress
- stem cells
- oxidative stress
- type diabetes
- magnetic resonance imaging
- cell death
- dna methylation
- skeletal muscle
- single cell
- metabolic syndrome
- fatty acid