980nm Photobiomodulation Promotes Osteo/odontogenic Differentiation of the Stem Cells from Human Exfoliated Deciduous Teeth via the Cross-talk between BMP/Smad and Wnt/β-catenin Signaling Pathways.
Ziting WangTian TianLeyi ChenChuhan ZhangXiao ZhengNi ZhongBuling WuWen'an XuPublished in: Photochemistry and photobiology (2022)
Increasing evidence suggests stem cells from human exfoliated deciduous teeth (SHEDs) serve as desirable sources of dentin regeneration. Photobiomodulation (PBM) has shown great potential in enhancing the proliferation and osteogenesis of human bone marrow mesenchymal stem cells (hBMMSCs). However, the specific role of PBM in odontogenic differentiation of SHEDs is little know, and we further investigated potential mechanism of PBM osteo/odontogenisis. A 980 nm diode laser with different energy densities of (0.5, 5, 10 J/cm 2 ) in a 100-mW continuous wave was used for irradiation every 24h. Osteo/odontogenic differentiation of SHEDs was achieved by performing alkaline phosphatase (ALP) and alizarin red staining (ARS) and osteo/odontogenic markers were also evaluated by qRT-PCR and western blotting. Additionally, western blot and immunohistochemical staining were performed to evaluate the levels of BMP/Smad and Wnt/β-catenin signaling-related proteins. We found that PBM at 5 J/cm 2 increased mineral deposition, and upregulated the expression of related osteo/odontogenic markers along with the elevated expression of β-catenin and phosphorylation level of Smad1/5/9. Furthermore, Wnt signaling inhibition using DKK1 and BMP signaling inhibition using noggin inhibited PBM-induced osteo/odontogenic marker expression when used individually or jointly. In conclusion, PBM induces the osteo/odontogenic differentiation of SHEDs through cross-talk between BMP/Smad and Wnt/β-catenin signaling pathways.
Keyphrases
- epithelial mesenchymal transition
- cell proliferation
- signaling pathway
- endothelial cells
- poor prognosis
- transforming growth factor
- stem cells
- mesenchymal stem cells
- induced pluripotent stem cells
- bone regeneration
- pluripotent stem cells
- high glucose
- south africa
- pi k akt
- binding protein
- radiation therapy
- long non coding rna
- risk assessment
- bone marrow
- drug induced
- oxidative stress
- mass spectrometry
- protein kinase
- real time pcr