The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.
Xilin LiYuxi LiKylie G NingSi ChenLei GuoJessica A BonzoNan MeiPublished in: Journal of environmental science and health. Part C, Toxicology and carcinogenesis (2022)
In vitro genotoxicity testing plays an important role in chemical risk assessment. The human B-lymphoblastoid cell line TK6 is widely used as a standard cell line for regulatory safety evaluations. Like many other mammalian cell lines, TK6 cells have limited metabolic capacity; therefore, usually require a source of exogenous metabolic activation for use in genotoxicity testing. Previously, we developed a set of TK6-derived cell lines that individually express one of fourteen cytochrome P450s (CYPs). In the present study, we surveyed a panel of major Phase II drug-metabolizing enzymes to characterize their baseline expression in TK6 cells. These results may serve as a reference enzymatic profile of this commonly used cell line.
Keyphrases
- phase ii
- induced apoptosis
- clinical trial
- cell cycle arrest
- risk assessment
- open label
- endothelial cells
- poor prognosis
- randomized controlled trial
- transcription factor
- nitric oxide
- induced pluripotent stem cells
- hydrogen peroxide
- phase iii
- long non coding rna
- binding protein
- drug induced
- double blind
- climate change
- study protocol