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Construction of a dual-functional dumbbell probe-based fluorescent biosensor for cascade amplification detection of miRNAs in lung cancer cells and tissues.

Qian ZhangYun HanChen-Chen LiXiaoran ZouFei MaShuangshuang Zhang
Published in: Chemical communications (Cambridge, England) (2022)
We develop a dual-functional dumbbell probe-based fluorescent biosensor for cascade amplification detection of miRNAs in lung cancer cells and tissues by integrating a primer exchange reaction (PER) with the CRISPR-Cas12a system. This biosensor can absolutely quantify the miR-486-5p expression in different lung cancer cells and distinguish non-small cell lung cancer (NSCLC) patients from healthy individuals, holding great potential in biomedical research and clinical diagnosis.
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