Construction of a dual-functional dumbbell probe-based fluorescent biosensor for cascade amplification detection of miRNAs in lung cancer cells and tissues.
Qian ZhangYun HanChen-Chen LiXiaoran ZouFei MaShuangshuang ZhangPublished in: Chemical communications (Cambridge, England) (2022)
We develop a dual-functional dumbbell probe-based fluorescent biosensor for cascade amplification detection of miRNAs in lung cancer cells and tissues by integrating a primer exchange reaction (PER) with the CRISPR-Cas12a system. This biosensor can absolutely quantify the miR-486-5p expression in different lung cancer cells and distinguish non-small cell lung cancer (NSCLC) patients from healthy individuals, holding great potential in biomedical research and clinical diagnosis.
Keyphrases
- label free
- quantum dots
- crispr cas
- end stage renal disease
- living cells
- sensitive detection
- gene expression
- chronic kidney disease
- small cell lung cancer
- newly diagnosed
- ejection fraction
- poor prognosis
- genome editing
- loop mediated isothermal amplification
- prognostic factors
- gold nanoparticles
- nucleic acid
- binding protein
- climate change
- brain metastases
- high density