Injection Vaccines Formulated with Nucleotide, Liposomal or Mineral Oil Adjuvants Induce Distinct Differences in Immunogenicity in Rainbow Trout.
Kasper Rømer VillumsenPer Walter KaniaDennis ChristensenErling Olaf KoppangAnders Miki BojesenPublished in: Vaccines (2020)
Protection facilitated by the widespread use of mineral oil adjuvanted injection vaccines in salmonid fish comes with adverse effects of varying severity. In this study, we characterized the immunological profiles of two alternative vaccine formulations, both with proven efficacy and an improved safety profile in rainbow trout. Experimental injection vaccines were prepared on an identical whole-cell Aeromonas salmonicida bacterin platform and were formulated with CpG oligodeoxynucleotides, a liposomal (CAF01) or a benchmark mineral oil adjuvant, respectively. A naïve group, as well as bacterin and saline-injected groups were also included. Following administration, antigen-specific serum antibody titers, the tissue distribution of immune cell markers, and the expression of immune-relevant genes following the in vitro antigenic restimulation of anterior kidney leukocytes was investigated. Immunohistochemical staining suggested prolonged antigen presentation for the particulate formulations and increased mucosal presence of antigen-presenting cells in all immunized fish. Unlike the other immunized groups, the CAF01 group only displayed a transient elevation in specific antibody titers and immunohistochemical observations, and the transcription data suggest an increased role of cell-mediated immunity for this group. Finally, the transcription profile of the CpG formulation approached that of a TH1 profile. When compared to the benchmark formulation, CAF01 and CpG adjuvants induce slight, but distinct differences in the resulting protective immune responses. This is important, as it allows a broader immunological approach for the future development of safer vaccines.
Keyphrases
- dna methylation
- immune response
- single cell
- ultrasound guided
- cell therapy
- induced apoptosis
- drug delivery
- poor prognosis
- fatty acid
- transcription factor
- early stage
- genome wide
- stem cells
- emergency department
- cell cycle arrest
- toll like receptor
- signaling pathway
- dendritic cells
- electronic health record
- current status
- inflammatory response
- machine learning
- mesenchymal stem cells
- peripheral blood
- long non coding rna
- high resolution
- blood brain barrier
- single molecule
- subarachnoid hemorrhage
- flow cytometry
- bone marrow