Efficacy of Hydroxytyrosol-Rich Food Supplements on Reducing Lipid Oxidation in Humans.
Cecilia BenderIlaria CandiEva RogelPublished in: International journal of molecular sciences (2023)
In the present study we report the efficacy of two food supplements derived from olives in reducing lipid oxidation. To this end, 12 healthy volunteers received a single dose (25 mL) of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), followed by an investigation of two reliable markers of oxidative stress. Blood and urine samples were collected at baseline and at 0.5, 1, 1.5, 2, 4, and 12 h post-intake. Plasma-oxidized low-density lipoprotein (oxLDL) cholesterol levels were measured with ELISA using a monoclonal antibody, while F2-isoprostanes (F2-IsoPs) were quantified in urine with UHPLC-DAD-MS/MS. Despite the great variability observed between individuals, a tendency to reduce lipoxidation reactions was observed in the blood in response to a single intake of the food supplements. In addition, the subgroup of individuals with the highest baseline oxLDL level showed a significant ( p < 0.05) decrease in F2-IsoPs at 0.5 and 12 h post-intervention. These promising results suggest that HT supplementation could be a useful aid in preventing lipoxidation. Additionally, people with a redox imbalance could benefit even more from supplementing with bioavailable HT.
Keyphrases
- low density lipoprotein
- ms ms
- monoclonal antibody
- oxidative stress
- randomized controlled trial
- liquid chromatography tandem mass spectrometry
- human health
- simultaneous determination
- dna damage
- clinical trial
- fatty acid
- induced apoptosis
- risk assessment
- ischemia reperfusion injury
- ionic liquid
- diabetic rats
- ultra high performance liquid chromatography
- phase iii
- heat shock protein
- high resolution mass spectrometry