Deciphering the microbiome shift during fermentation of medicinal plants.
Martina KöberlSabine ErschenMohammad EtemadiRichard Allen WhiteTarek F El-ArabiGabriele BergPublished in: Scientific reports (2019)
The importance of the human-microbiome relationship for positive health outcomes has become more apparent over the last decade. Influencing the gut microbiome via modification of diet represents a possibility of maintaining a healthy gut flora. Fermented food and lactic acid bacteria (LAB) display a preventive way to inhibit microbial dysbioses and diseases, but their ecology on plants is poorly understood. We characterized the microbiome of medicinal plants (Matricaria chamomilla L. and Calendula officinalis L.) using 16S rRNA gene profiling from leaves that were fermented over a six-week time course. The unfermented samples were characterized by a distinct phyllosphere microbiome, while the endosphere revealed a high similarity. During fermentation, significant microbial shifts were observed, whereby LAB were enhanced in all approaches but never numerically dominated. Among the LAB, Enterococcaceae were identified as the most dominant family in both plants. M. chamomilla community had higher relative abundances of Lactobacillaceae and Carnobacteriaceae, while C. officinalis showed a higher presence of Leuconostocaceae and Streptococcaceae. The natural leaf microbiome and the indigenous LAB communities of field-grown Asteraceae medicinal plants are plant-specific and habitat-specific and are subjected to significant shifts during fermentation. Leaf surfaces as well as leaf endospheres were identified as sources for biopreservative LAB.
Keyphrases
- lactic acid
- endothelial cells
- healthcare
- saccharomyces cerevisiae
- physical activity
- gene expression
- drinking water
- single cell
- magnetic resonance
- climate change
- risk assessment
- magnetic resonance imaging
- clinical trial
- escherichia coli
- cystic fibrosis
- human health
- pseudomonas aeruginosa
- candida albicans
- contrast enhanced
- pluripotent stem cells
- cell wall