Effect of angiotensin II and angiotensin-(1-7) on proliferation of stem cells from human dental apical papilla.
Larissa M MacedoRenato I de ÁvilaGustavo R PedrinoDiego B ColugnatiMarize C ValadaresEliana M LimaClayton L BorgesGregory T KittenElisandra GavaCarlos Henrique de CastroPublished in: Journal of cellular physiology (2020)
The effects of the renin-angiotensin system (RAS) on stem cells isolated from human dental apical papilla (SCAPs) are completely unknown. Therefore, the aim of this study was to identify RAS components expressed in SCAPs and the effects of angiotensin (Ang) II and Ang-(1-7) on cell proliferation. SCAPs were collected from third molar teeth of adolescents and maintained in cell culture. Messenger RNA expression and protein levels of angiotensin-converting enzyme (ACE), ACE2, and Mas, Ang II type I (AT1) and type II (AT2) receptors were detected in SCAPs. Treatment with either Ang II or Ang-(1-7) increased the proliferation of SCAPs. These effects were inhibited by PD123319, an AT2 antagonist. While Ang II augmented mTOR phosphorylation, Ang-(1-7) induced ERK1/2 phosphorylation. In conclusion, SCAPs produce the main RAS components and both Ang II and Ang-(1-7) treatments induced cell proliferation mediated by AT2 activation through different intracellular mechanisms.
Keyphrases
- angiotensin ii
- angiotensin converting enzyme
- cell proliferation
- stem cells
- vascular smooth muscle cells
- endothelial cells
- signaling pathway
- high glucose
- cell cycle
- pi k akt
- young adults
- diabetic rats
- small molecule
- poor prognosis
- long non coding rna
- oral health
- protein kinase
- smoking cessation
- pluripotent stem cells
- amino acid