Zearalenone Promotes Uterine Hypertrophy through AMPK/mTOR Mediated Autophagy.
Li-Jie YangWenshuang LiaoJiuyuan DongXiangjin ChenLibo HuangWeiren YangShu Zhen JiangPublished in: Toxins (2024)
Zearalenone (ZEN), a non-steroidal Fusarium graminearum with an estrogen effect, can cause damage to the gastrointestinal tract, immune organs, liver, and reproductive system. Further analysis of the mechanism of ZEN has become an important scientific issue. We have established in vivo and in vitro models of ZEN intervention, used AMPK/mTOR as a targeted pathway for ZEN reproductive toxicity, and explored the molecular mechanism by which ZEN may induce uterine hypertrophy in weaned piglets. Our study strongly suggested that ZEN can activate the phosphorylation of AMPK in uterine endometrial epithelium cells, affect the phosphorylation level of mTOR through TSC2 and Rheb, induce autophagy, upregulate the expression of proliferative genes PCNA and BCL2, downregulate the expression of apoptotic gene BAX, promote uterine endometrial epithelium cells proliferation, and ultimately lead to thickening of the endometrial and myometrium, increased density of uterine glands, and induce uterine hypertrophy.
Keyphrases
- induced apoptosis
- cell death
- oxidative stress
- endoplasmic reticulum stress
- signaling pathway
- cell cycle arrest
- protein kinase
- poor prognosis
- skeletal muscle
- cell proliferation
- randomized controlled trial
- genome wide
- endometrial cancer
- dna methylation
- genome wide identification
- copy number
- transcription factor
- anti inflammatory
- estrogen receptor
- genome wide analysis