The Prevention Role of Theaflavin-3,3'-digallate in Angiotensin II Induced Pathological Cardiac Hypertrophy via CaN-NFAT Signal Pathway.
Hui ZhouChen XiaYaqing YangHasitha Kalhari WarusawitharanaXiaohui LiuYouying TuPublished in: Nutrients (2022)
Theaflavin-3,3'-digallate (TF3) is a representative theaflavin of black tea and is remarkable for the anti-coronary heart disease effect. As an adaptive response to heart failure, pathological cardiac hypertrophy (PCH) has attracted great interest. In this study, the PCH cell model was established with H9c2 cells by angiotensin II, and the prevention effect and mechanisms of TF3 were investigated. The results showed that the cell size and fetal gene mRNA level were significantly reduced as pretreated with TF3 at the concentration range of 1-10 μM, also the balance of the redox system was recovered by TF3 at the concentration of 10 μM. The intracellular Ca 2+ level decreased, Calcineurin (CaN) expression was down-regulated and the p-NFATc3 expression was up-regulated. These results indicated that TF3 could inhibit the activation of the CaN-NFAT signal pathway to prevent PCH, and TF3 may be a potentially effective natural compound for PCH and heart failure.
Keyphrases
- angiotensin ii
- heart failure
- angiotensin converting enzyme
- vascular smooth muscle cells
- poor prognosis
- single cell
- induced apoptosis
- binding protein
- transcription factor
- left ventricular
- genome wide
- stem cells
- gene expression
- cross sectional
- atrial fibrillation
- cell proliferation
- immune response
- cardiac resynchronization therapy
- cell death
- dna methylation
- mass spectrometry
- high glucose
- oxidative stress
- high resolution
- endoplasmic reticulum stress
- toll like receptor