Development and validation of an HPLC-DAD method for determination of oleuropein and other bioactive compounds in olive leaf by-products.

Esther M Martínez-NavarroCristina Cebrián-TarancónNatalia Moratalla-LópezCándida LorenzoGonzalo Luis Alonso María Rosario Salinas

Published in: Journal of the science of food and agriculture (2020)

A simple extraction method was developed and validated to obtain aqueous extract from olive leaves by microwave extraction, determining for the first time oleuropein water solubility. Validation of the method showed that oleuropein in olive leaves could be quantified when it is at least 1% of dry weight by means of HPLC-DAD. UV-visible spectrophotometry can be useful in oil mills because it enables the content of oleuropein and other bioactive compounds content to be determined in situ in such leaf aqueous extracts. © 2020 Society of Chemical Industry.

Keyphrases

ms ms
simultaneous determination
solid phase extraction
high performance liquid chromatography
ionic liquid
mass spectrometry
tandem mass spectrometry
oxidative stress
body mass index
physical activity
weight loss
liquid chromatography
anti inflammatory
essential oil
body weight
radiofrequency ablation