Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.
Luis Felipe ClementeMaría Luisa HernáezAntonio Ramos-FernándezGertrudis LigeroConcha GilFernando José CorralesMiguel MarcillaPublished in: Journal of proteome research (2018)
Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.
Keyphrases
- mesenchymal stem cells
- umbilical cord
- adipose tissue
- endothelial cells
- bone marrow
- mass spectrometry
- induced pluripotent stem cells
- cell therapy
- pluripotent stem cells
- type diabetes
- poor prognosis
- machine learning
- binding protein
- liquid chromatography
- pet imaging
- loop mediated isothermal amplification
- long non coding rna