Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage.

Suleiman Alhaji MuhammadNorshariza NordinPaisal HussinMuhammad Zulfadli MehatSheau Wei TanSharida Fakurazi

Published in: Cartilage (2019)

Collagenase in HBSS yield the highest number of viable chondrocytes and the isolated cells expressed chondrocyte phenotype. This protocol can be employed to generate large number of viable chondrocytes, particularly with limited cartilage biopsies.

Keyphrases

extracellular matrix
induced apoptosis
randomized controlled trial
endothelial cells
cell cycle arrest
signaling pathway
pluripotent stem cells
oxidative stress
cell death
pi k akt