Tracking Stress Granule Dynamics in Live Cells and In Vivo with a Small Molecule.
Wen ShaoShu-Tang ZengZe-Yi YuGui-Xue TangShuo-Bin ChenShuo-Bin ChenXiu-Cai ChenJia-Heng TanPublished in: Analytical chemistry (2021)
Because of the lack of facile and accurate methods to track stress granule (SG) dynamics in live cells and in vivo, in-depth studies of the biological roles of this attractive membraneless organelle have been limited. Herein, we report the first small-molecule probe, TASG, for the selective, convenient and real-time monitoring of SGs. This novel molecule can simultaneously bind to SG RNAs, the core SG protein G3BP1, and their complexes, triggering a significant enhancement in fluorescence intensity, making TASG broadly applicable to SG imaging under various stress conditions in fixed and live cells, ex vivo and in vivo. Using TASG, the complicated endogenous SG dynamics were revealed in both live cells and C. elegans. Collectively, our work provides an ideal probe that has thus far been absent in the field of SG investigations. We anticipate that this powerful tool may create exciting opportunities to investigate the underlying roles of SGs in different organisms.
Keyphrases
- induced apoptosis
- small molecule
- cell cycle arrest
- endoplasmic reticulum stress
- high resolution
- quantum dots
- signaling pathway
- cell death
- oxidative stress
- single molecule
- high intensity
- photodynamic therapy
- heat stress
- stress induced
- binding protein
- gold nanoparticles
- gram negative
- highly efficient
- fluorescence imaging