Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway.
Mengfan DingBowen LiHaiqin ChenDong LiangR Paul RossCatherine StantonJianxin ZhaoWei ChenChen WeiPublished in: Gut microbes (2023)
Bifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM11269 group (administered B. longum subsp. infantis CCFM1269, 1 × 10 9 CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 × 10 9 CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.
Keyphrases
- endothelial cells
- high fat diet induced
- signaling pathway
- randomized controlled trial
- clinical trial
- poor prognosis
- type diabetes
- growth hormone
- genome wide
- ms ms
- metabolic syndrome
- mass spectrometry
- binding protein
- cell proliferation
- pi k akt
- adipose tissue
- bone mineral density
- skeletal muscle
- bioinformatics analysis
- genome wide identification