The emerging landscape of single-molecule protein sequencing technologies.
Javier Antonio AlfaroPeggy BohländerMingjie DaiMike FiliusCecil J HowardXander F van KootenShilo OhayonAdam PomorskiSonja SchmidAleksei AksimentievEric V AnslynGeorges BedranChan CaoMauro ChinappiEtienne CoyaudCees DekkerGunnar DittmarNicholas DrachmanRienk EelkemaDavid GoodlettSébastien HentzUmesh KalathiyaMichael P SnyderRyan T KellyZvi KelmanSung Hyun KimBernhard KusterDavid Rodriguez-LarreaStuart LindsayGiovanni MagliaEdward M MarcotteJohn P MarinoChristophe D MasselonMichael MayerPatroklos SamarasKumar SarthakLusia SepiashviliDerek SteinMeni WanunuMathias WilhelmPeng YinAmit MellerChirlmin JooPublished in: Nature methods (2021)
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.