5-Aminolevulinic acid production from inexpensive glucose by engineering the C4 pathway in Escherichia coli.
Wenwen DingHuanjiao WengGuocheng DuJian ChenZhen KangPublished in: Journal of industrial microbiology & biotechnology (2017)
5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L. Following optimization of biosynthesis pathways towards coenzyme A and precursor (glycine and succinyl-CoA), and downregulation of hemB expression, the production of ALA was further increased to 2.81 g/L in batch-fermentation.
Keyphrases
- escherichia coli
- photodynamic therapy
- blood glucose
- poor prognosis
- biofilm formation
- klebsiella pneumoniae
- cell wall
- working memory
- cell proliferation
- copy number
- genome wide
- fatty acid
- binding protein
- saccharomyces cerevisiae
- cystic fibrosis
- gene expression
- adipose tissue
- skeletal muscle
- metabolic syndrome
- cell free
- genome wide identification