Maternal ageing causes changes in DNA methylation and gene expression profiles in mouse oocytes.
Guan-Mei HouQing-Yuan SunPublished in: Zygote (Cambridge, England) (2020)
Although it is well known that maternal ageing causes reduced oocyte quality and fertility, little information is known about its effect on germ cell epigenetics. In the present study, we compared the gene expression and DNA methylation profiles in germinal vesicle oocytes from young (8-week-old) and aged (18-month-old) mice using single-cell RNA-sequencing and single-cell whole-genome DNA methylation sequencing. We found significant differences in the data from the two groups. Oocytes from aged mice showed significant changes in the expression of some metabolism-related genes, such as mitochondria-associated genes, that was in line with our expectations. Expression of some genes associated with reproduction also showed significant differences. DNA methylation levels were also changed in oocytes from aged mice. The two groups had significant gaps in hypermethylation and hypomethylation levels on each chromosome. These data provide useful information for further understanding the mechanisms of oocyte ageing.
Keyphrases
- dna methylation
- single cell
- gene expression
- genome wide
- rna seq
- poor prognosis
- high fat diet induced
- copy number
- germ cell
- high throughput
- electronic health record
- health information
- big data
- randomized controlled trial
- machine learning
- cell death
- pregnant women
- metabolic syndrome
- binding protein
- wild type
- physical activity
- insulin resistance
- adipose tissue
- healthcare
- artificial intelligence
- young adults
- quality improvement
- genome wide identification
- weight loss