Multiwalled Carbon Nanotubes Alter the PSII Photochemistry, Photosystem-Related Gene Expressions, and Chloroplastic Antioxidant System in Zea mays under Copper Toxicity.

A critical approach against copper (Cu) toxicity is the use of carbon nanomaterials (CNMs). However, the effect of CNMs on Cu toxicity-exposed chloroplasts is not clear. The photosynthetic, genetic, and biochemical effects of multiwalled carbon nanotubes (50-100-250 mg L -1 CNT) were investigated under Cu stress (50-100 μM CuSO 4 ) in Zea mays chloroplasts. F v /F m and F v /F o were suppressed under stress. Stress altered the antioxidant system and the expression of psaA , psaB , psbA , and psbD . The chloroplastic activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione S-transferase (GST), and glutathione peroxidase (GPX) increased under CNT + stress, and those of hydrogen peroxide (H 2 O 2 ) and lipid peroxidation decreased. CNTs were promoted to the maintenance of the redox state by regulating enzyme/non-enzyme activity/contents involved in the AsA-GSH cycle. Furthermore, CNTs inverted the negative effects of Cu by upregulating the transcriptions of photosystem-related genes. However, the high CNT concentration had adverse effects on the antioxidant capacity. CNT has great potential to confer tolerance by reducing Cu-induced damage and protecting the biochemical reactions of photosynthesis.