Improved analysis of CRISPR fitness screens and reduced off-target effects with the BAGEL2 gene essentiality classifier.

Published in: Genome medicine (2021)

BAGEL2 substantially improves the sensitivity, specificity, and performance over BAGEL and establishes the new state of the art in the analysis of CRISPR knockout fitness screens. BAGEL2 is written in Python 3 and source code, along with all supporting files, are available on github ( https://github.com/hart-lab/bagel ).

Keyphrases

genome wide
dna methylation
body composition
physical activity
copy number
genome editing
high throughput
gene expression
single cell
transcription factor
wild type