Oxidation-guided and collision-induced linearization assists de novo sequencing of thioether macrocyclic peptides.
Ayaka HayashiYuki GotoYutaro SaitoHiroaki SugaJumpei MorimotoShinsuke SandoPublished in: Chemical communications (Cambridge, England) (2024)
Oxidation of a thioether linkage in thioether-closed macrocyclic peptides led to collision-induced site-selective linearization of the peptides. This method has allowed for de novo sequencing of thioether macrocyclic peptides. The utility of the sequencing method was demonstrated by identifying the correct peptide sequences from a virtually randomized thioether macrocyclic peptide library.
Keyphrases
- single cell
- high glucose
- amino acid
- diabetic rats
- hydrogen peroxide
- drug induced
- double blind
- clinical trial
- nitric oxide
- randomized controlled trial
- endothelial cells
- genome wide
- gene expression
- placebo controlled
- phase ii
- human immunodeficiency virus
- hiv infected
- men who have sex with men
- high throughput sequencing