Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci.
Weiqian LiYue HuoYue RenChenxi HanShuo LiKangning WangManman HeYiying ChenYanran WangLingjie XuYuehong GuoYanmin SiYufeng GaoJiayue XuXiaoshuang WangYanni MaJia YuFang WangPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2023)
Albeit the majority of eukaryotic genomes can be pervasively transcribed to a diverse population of lncRNAs and various subtypes of lncRNA are discovered. However, the genome-wide study of miRNA-derived lncRNAs is still lacking. Here, it is reported that over 800 miRNA gene-originated lncRNAs (molncRNAs) are generated from miRNA loci. One of them, molnc-301b from miR-301b and miR-130b, functions as an "RNA decoy" to facilitate dissociation of the chromatin remodeling protein SMARCA5 from chromatin and thereby sequester transcription and mRNA translation. Specifically, molnc-301b attenuates erythropoiesis by mitigating the transcription of erythropoietic and translation-associated genes, such as GATA1 and FOS. In addition, a useful and powerful CRISPR screen platform to characterize the biological functions of molncRNAs at large-scale and single-cell levels is established and 29 functional molncRNAs in hematopoietic cells are identified. Collectively, the focus is on miRNA-derived lncRNAs, deciphering their landscape during normal hematopoiesis, and comprehensively evaluating their potential roles.
Keyphrases
- genome wide
- genome wide identification
- long non coding rna
- transcription factor
- dna methylation
- single cell
- copy number
- high throughput
- genome wide analysis
- network analysis
- induced apoptosis
- poor prognosis
- rna seq
- gene expression
- binding protein
- cell cycle arrest
- risk assessment
- dna damage
- oxidative stress
- genome editing
- crispr cas
- long noncoding rna
- amino acid