Laser capture microdissection for transcriptomic profiles in human skin biopsies.

Silvia SantoroIgnazio Diego LopezRaffaella LombardiAndrea ZauliAna Maria OsiceanuMelissa SorosinaFerdinando ClarelliSilvia PeroniDaniele CazzatoMargherita MarchiAngelo QuattriniGiancarlo ComiRaffaele Adolfo CalogeroGiuseppe LauriaFilippo Martinelli Boneschi

Published in: BMC molecular biology (2018)

The described implemented and optimized protocol can be used for generating transcriptomics data on skin tissues, and it is potentially applicable to other tissues. It can be extended to multicenter studies, due to the introduction of an initial step of preservation of the specimen that allowed the shipment of biological samples.

Keyphrases

single cell
gene expression
randomized controlled trial
rna seq
electronic health record
soft tissue
cross sectional
wound healing
clinical trial
case control
machine learning
mass spectrometry
ultrasound guided