Light-emitting diode irradiation induces AKT/mTOR-mediated apoptosis in human pancreatic cancer cells and xenograft mouse model.
Young Mi KimSung Hwa KoYong Il ShinYeonye KimTaehyung KimJaehoon JungSang-Yull LeeNam Gyun KimKyoung-Jun ParkJi Hyeon RyuPublished in: Journal of cellular physiology (2020)
The beneficial effects of light-emitting diode (LED) irradiation have been reported in various pathologies, including cancer. However, its effect in pancreatic cancer cells remains unclear. Herein, we demonstrated that blue LED of 460 nm regulated pancreatic cancer cell proliferation and apoptosis by suppressing the expression of apoptosis-related factors, such as mutant p53 and B-cell lymphoma 2 (Bcl-2), and decreasing the expression of RAC-β serine/threonine kinase 2 (AKT2), the phosphorylation of protein kinase B (AKT), and mammalian target of rapamycin (mTOR). Blue LED irradiation also increased the levels of cleaved poly-(ADP-ribose) polymerase (PARP) and caspase-3 in pancreatic cancer cells, while it suppressed AKT2 expression and inhibited tumor growth in xenograft tumor tissues. In conclusion, blue LED irradiation suppressed pancreatic cancer cell and tumor growth by regulating AKT/mTOR signaling. Our findings indicated that blue LEDs could be used as a nonpharmacological treatment for pancreatic cancer.
Keyphrases
- light emitting
- cell proliferation
- protein kinase
- poor prognosis
- signaling pathway
- cell cycle
- cell death
- pi k akt
- mouse model
- oxidative stress
- cell cycle arrest
- endoplasmic reticulum stress
- binding protein
- dna damage
- long non coding rna
- transcription factor
- dna repair
- gene expression
- squamous cell carcinoma
- diffuse large b cell lymphoma
- tyrosine kinase
- combination therapy
- young adults
- wild type
- pluripotent stem cells