MECAT: fast mapping, error correction, and de novo assembly for single-molecule sequencing reads.

Chuan-Le XiaoYing ChenShang-Qian XieKai-Ning ChenYan WangYue HanFeng Luo Zhi Xie

Published in: Nature methods (2017)

We present a tool that combines fast mapping, error correction, and de novo assembly (MECAT; accessible at https://github.com/xiaochuanle/MECAT) for processing single-molecule sequencing (SMS) reads. MECAT's computing efficiency is superior to that of current tools, while the results MECAT produces are comparable or improved. MECAT enables reference mapping or de novo assembly of large genomes using SMS reads on a single computer.

Keyphrases

single molecule
high resolution
atomic force microscopy
living cells
high density
deep learning
mass spectrometry