CRISPR/Cas9 mediated GFP-human dentin matrix protein 1 (DMP1) promoter knock-in at the ROSA26 locus in mesenchymal stem cell for monitoring osteoblast differentiation.

Fahimeh ShahabipourReza Kazemi OskueeMohammad Ali ShokrgozarHojjat Naderi-MeshkinLena GoshayeshiFariba Ghalamkarpour

Published in: The journal of gene medicine (2020)

The data show that DMP1-DsRed-GFP knock-in MSCs through CRISPR/Cas9 technology provide a valuable indicator for osteoblast differentiation. Moreover, The DMP1 promoter might be used as a predictive marker of MSCs differentiated toward osteoblasts.

Keyphrases

crispr cas
mesenchymal stem cells
genome editing
umbilical cord
dna methylation
endothelial cells
transcription factor
gene expression
bone marrow
electronic health record
induced pluripotent stem cells
pluripotent stem cells
big data
binding protein
data analysis
genome wide association study