Antitumor effect of TW-37, a BH3 mimetic in human oral cancer.
Chi-Hyun AhnWon Woo LeeYun Chan JungJi-Ae ShinKyoung-Ok HongSujung ChoiNeeti SwarupJihoon KimMin-Hye AhnMinjung JungSung-Dae ChoBohwan JinPublished in: Laboratory animal research (2019)
TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer. TW-37 significantly inhibited cell proliferation and increased the number of dead cells in MC-3 and HSC-3 human oral cancer cell lines. TW-37 enhanced apoptosis of both cell lines evidenced by annexin V/propidium iodide double staining, sub-G1 population analysis and the detection of cleaved poly (ADP-ribose) polymerase and caspase-3. In addition, TW-37 markedly downregulated the expression of Bcl-2 protein, while not affecting Bcl-xL or myeloid cell leukemia-1. In vivo, TW-37 inhibited tumor growth in a nude mice xenograft model without any significant liver and kidney toxicities. Collectively, these data reveal that TW-37 may be a promising small molecule to inhibit human oral cancer.
Keyphrases
- endothelial cells
- small molecule
- induced pluripotent stem cells
- cell proliferation
- pluripotent stem cells
- bone marrow
- oxidative stress
- acute myeloid leukemia
- induced apoptosis
- stem cells
- cell cycle arrest
- type diabetes
- cell death
- protein protein
- adipose tissue
- genome wide
- metabolic syndrome
- dna methylation
- machine learning
- single cell
- binding protein
- poor prognosis
- cell cycle
- endoplasmic reticulum stress
- immune response
- gene expression
- deep learning
- long non coding rna
- pi k akt