Flavonoids-Enriched Vegetal Extract Prevents the Activation of NFκB Downstream Mechanisms in a Bowel Disease In Vitro Model.
Paolo CorbettaElena LonatiStefania PagliariMario MauriEmanuela CazzanigaLaura BottoLuca CamponePaola PalestiniAlessandra BulbarelliPublished in: International journal of molecular sciences (2024)
Inflammatory bowel disease (IBD) incidence has increased in the last decades due to changes in dietary habits. IBDs are characterized by intestinal epithelial barrier disruption, increased inflammatory mediator production and excessive tissue injury. Since the current treatments are not sufficient to achieve and maintain remission, complementary and alternative medicine (CAM) becomes a primary practice as a co-adjuvant for the therapy. Thus, the intake of functional food enriched in vegetal extracts represents a promising nutritional strategy. This study evaluates the anti-inflammatory effects of artichoke, caihua and fenugreek vegetal extract original blend (ACFB) in an in vitro model of gut barrier mimicking the early acute phases of the disease. Caco2 cells cultured on transwell supports were treated with digested ACFB before exposure to pro-inflammatory cytokines. The pre-treatment counteracts the increase in barrier permeability induced by the inflammatory stimulus, as demonstrated by the evaluation of TEER and CLDN-2 parameters. In parallel, ACFB reduces p65NF-κB pro-inflammatory pathway activation that results in the decrement of COX-2 expression as PGE2 and IL-8 secretion. ACFB properties might be due to the synergistic effects of different flavonoids, indicating it as a valid candidate for new formulation in the prevention/mitigation of non-communicable diseases.
Keyphrases
- anti inflammatory
- oxidative stress
- induced apoptosis
- signaling pathway
- lps induced
- ulcerative colitis
- endothelial cells
- weight gain
- primary care
- liver failure
- healthcare
- nuclear factor
- climate change
- pi k akt
- risk factors
- early stage
- respiratory failure
- drug induced
- human health
- systemic lupus erythematosus
- intensive care unit
- disease activity
- inflammatory response
- cancer therapy
- cell proliferation
- risk assessment
- stem cells
- acute respiratory distress syndrome
- toll like receptor
- immune response
- combination therapy
- heavy metals
- long non coding rna
- anaerobic digestion