Nelumbinis Stamen Ameliorates Chronic Restraint Stress-Induced Muscle Dysfunction and Fatigue in Mice by Decreasing Serum Corticosterone Levels and Activating Sestrin2.
Zhi WangSuwei JinTianji XiaYongguang LiuYunfeng ZhouXin-Min LiuRuile PanYong-Hong LiaoMingzhu YanQi ChangPublished in: Journal of agricultural and food chemistry (2022)
Nelumbo nucifera Gaertn. is an important aquatic vegetable, and its dried stamen (Nelumbinis stamen, NS) is a valuable nutraceutical usually used as a herbal tea. Here, we used ultrahigh-performance liquid chromatography (UPLC)-quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography (HPLC) to chemically profile NS and quantify their main constituent flavonoids, respectively. In total, 44 components were identified, including organic acids, flavonoids, monoterpene glycosides, and fatty acids. Experimental mice were induced with fatigue by exposure to chronic restraint stress (CRS) for 8 h daily for 15 days and then treated with an aqueous extract of NS (0.5 and 1 g/kg) via gavage. NS significantly mitigated CRS-induced skeletal muscle dysfunction and fatigue in mice possibly by lowering serum corticosterone levels and restoring Sestrin2 expression in the gastrocnemius to regulate metabolism, preserve mitochondrial homeostasis, and promote antioxidant capacity. These results demonstrate that NS can be used as a nutraceutical or supplement for controlling stress-induced muscle dysfunction and fatigue.
Keyphrases
- stress induced
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- skeletal muscle
- dengue virus
- liquid chromatography
- mass spectrometry
- oxidative stress
- solid phase extraction
- diabetic rats
- high fat diet induced
- sleep quality
- high glucose
- zika virus
- fatty acid
- insulin resistance
- drug induced
- high resolution mass spectrometry
- gas chromatography
- ms ms
- poor prognosis
- risk assessment
- aedes aegypti
- type diabetes
- high resolution
- metabolic syndrome
- endothelial cells
- signaling pathway
- anti inflammatory