Towards personalised anti-microbial and immune approaches to infections in acute care. Can real-time genomic-informed diagnosis of pathogens, and immune-focused therapies improve outcomes for patients? An observational, experimental study protocol.
Samuel QuartonKirsty McGeeNicola CumleyMahboobeh BehruzniaCharlotte JeffKylie BelchamberMichael J CoxDavid ThickettAaron ScottDhruv ParekhAlan McNallyElizabeth SapeyPublished in: PloS one (2024)
This is a prospective observational study of patients with acute infection. Patients with symptoms suggestive of an acute infection will be recruited, and blood and bodily fluid relevant to the site of infection collected (for example, sputum and naso-oropharyngeal swabs for respiratory tract infections, or urine for a suspected urinary tract infection). Metagenomic analysis of samples will be compared to traditional microbiology, alongside the antimicrobials received. Blood and respiratory samples such as bronchoalveolar lavage will be used to isolate immune cells and interrogate immune cell function. Where possible, similar samples will be collected from matched participants without a suspected infection to determine the impact of infection on both microbiome and immune cell function.
Keyphrases
- respiratory tract
- randomized controlled trial
- study protocol
- urinary tract infection
- acute care
- clinical trial
- mycobacterium tuberculosis
- end stage renal disease
- gene expression
- ejection fraction
- cystic fibrosis
- microbial community
- prognostic factors
- adipose tissue
- copy number
- multidrug resistant
- genome wide
- gram negative
- insulin resistance
- patient reported
- drug induced
- pulmonary tuberculosis
- extracorporeal membrane oxygenation
- placebo controlled