Klotho protein contributes to cardioprotection during ischaemia/reperfusion injury.
Agnieszka OlejnikAnna Krzywonos-ZawadzkaMarta BanaszkiewiczIwona Bil-LulaPublished in: Journal of cellular and molecular medicine (2020)
Restoration of blood flow to ischaemic heart inflicts ischaemia/reperfusion (I/R) injury, which manifests in metabolic and morphological disorders. Klotho is a protein with antioxidative and antiapoptotic activity, and is involved in the regulation of inflammation and fibrosis. The aim of the current research was to determine the role of Klotho in the heart subjected to I/R injury, as well as to study Klotho as a potential cardioprotective agent. Human cardiomyocytes and Wistar rat hearts perfused using Langendorff method subjected to I/R have been used. Hemodynamic parameters of heart function, markers of I/R injury, and gene and protein expression of Klotho were measured. Human cardiomyocytes were also incubated in the presence of recombinant Klotho protein, and the viability of cells was measured. There was a higher expression of Klotho gene and protein synthesis in the cardiomyocytes subjected to I/R injury. The compensatory production and release of Klotho protein from cardiac tissue during I/R were also shown. The treatment of cardiomyocytes subjected to I/R with Klotho protein resulted in increased viability and metabolic activity of cells. Thus, Klotho contributes to compensatory mechanism during I/R, and could be used as a marker of injury and as a potential cardiopreventive/cardioprotective agent.
Keyphrases
- blood flow
- endothelial cells
- induced apoptosis
- protein protein
- heart failure
- oxidative stress
- atrial fibrillation
- acute myocardial infarction
- poor prognosis
- genome wide
- gene expression
- copy number
- high glucose
- acute ischemic stroke
- cerebral ischemia
- signaling pathway
- induced pluripotent stem cells
- dna methylation
- long non coding rna
- acute coronary syndrome
- brain injury
- human health
- pluripotent stem cells