Cell proliferation and glial cell marker expression in the wall of the third ventricle in the tuberal region of the male mouse hypothalamus during postnatal development.
Adrian Coutteau-RoblesVincent PrevotAriane SharifPublished in: Journal of neuroendocrinology (2023)
The third ventricle (3 V) wall of the tuberal hypothalamus is composed of two types of cells; specialized ependymoglial cells called tanycytes located ventrally and ependymocytes dorsally, which control the exchanges between the cerebrospinal fluid and the hypothalamic parenchyma. By regulating the dialogue between the brain and the periphery, tanycytes are now recognized as central players in the control of major hypothalamic functions such as energy metabolism and reproduction. While our knowledge of the biology of adult tanycytes is progressing rapidly, our understanding of their development remains very incomplete. To gain insight into the postnatal maturation of the 3 V ependymal lining, we conducted a comprehensive immunofluorescent study of the mouse tuberal region at four postnatal ages (postnatal day (P) 0, P4, P10, and P20). We analyzed the expression profile of a panel of tanycyte and ependymocyte markers (vimentin, S100, connexin-43 [Cx43], and glial fibrillary acidic protein [GFAP]) and characterized cell proliferation in the 3 V wall using the thymidine analog bromodeoxyuridine. Our results show that most changes in marker expression occur between P4 and P10, with a switch from a 3 V mostly lined by radial cells to the emergence of a tanycytic domain ventrally and an ependymocytic domain dorsally, a drop in cell proliferation and increased expression of S100, Cx43, and GFAP that acquire a mature profile at P20. Our study thus identifies the transition between the first and the second postnatal week as a critical time window for the postnatal maturation of the 3 V wall ependymal lining.
Keyphrases
- cell proliferation
- preterm infants
- induced apoptosis
- poor prognosis
- cell cycle arrest
- pi k akt
- binding protein
- cerebrospinal fluid
- endoplasmic reticulum stress
- signaling pathway
- randomized controlled trial
- healthcare
- neuropathic pain
- pulmonary artery
- mitral valve
- oxidative stress
- gene expression
- heart failure
- stem cells
- single cell
- genome wide
- pulmonary hypertension
- clinical trial
- long non coding rna
- spinal cord injury
- small molecule
- atrial fibrillation
- resting state
- left ventricular
- coronary artery
- congenital heart disease
- ionic liquid
- spinal cord
- young adults
- study protocol