Mitigation of Deoxynivalenol (DON)- and Aflatoxin B1 (AFB1)-Induced Immune Dysfunction and Apoptosis in Mouse Spleen by Curcumin.
Azhar MuhmoodJianxin LiuDandan LiuShuiping LiuMahmoud Mostafa AzzamMuhammad Bilawal JunaidLili HouGuannan LeKehe HuangPublished in: Toxins (2024)
In the context of the potential immunomodulatory properties of curcumin in counteracting the detrimental effects of concurrent exposure to Deoxynivalenol (DON) and Aflatoxin B1 (AFB1), a comprehensive 28-days trial was conducted utilizing 60 randomly allocated mice divided into four groups. Administration of curcumin at a dosage of 5 mg/kg body weight in conjunction with DON at 0.1 mg/kg and AFB 1 at 0.01 mg/kg body weight was undertaken to assess its efficacy. Results indicated that curcumin intervention demonstrated mitigation of splenic structural damage, augmentation of serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels, elevation in T lymphocyte subset levels, and enhancement in the mRNA expression levels of pro-inflammatory cytokines TNF-α, IFN-γ, IL-2, and IL-6. Furthermore, curcumin exhibited a suppressive effect on apoptosis in mice, as evidenced by decreased activity of caspase-3 and caspase-9, reduced expression levels of pro-apoptotic markers Bax and Cytochrome-c (Cyt-c) at both the protein and mRNA levels, and the maintenance of a balanced expression ratio of mitochondrial apoptotic regulators Bax and Bcl-2. Collectively, these findings offer novel insights into the therapeutic promise of curcumin in mitigating immunosuppression and apoptotic events triggered by mycotoxin co-exposure.
Keyphrases
- cell death
- body weight
- oxidative stress
- cell cycle arrest
- anti inflammatory
- poor prognosis
- induced apoptosis
- randomized controlled trial
- endoplasmic reticulum stress
- binding protein
- rheumatoid arthritis
- climate change
- clinical trial
- squamous cell carcinoma
- small molecule
- high glucose
- high fat diet induced
- signaling pathway
- long non coding rna
- risk assessment
- peripheral blood
- rectal cancer
- open label
- artificial intelligence
- stress induced
- amino acid
- double blind