Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8.
Nan WangJing HeXiaoyu FengShengyou LiaoYi ZhaoFuchou TangKehkooi KeePublished in: eLife (2023)
Non-coding RNAs exert diverse functions in many cell types. In addition to transcription factors from coding genes, non-coding RNAs may also play essential roles in shaping and directing the fate of germ cells. The presence of many long non-coding RNAs (lncRNAs) which are specifically expressed in the germ cells during human gonadal development were reported and one divergent lncRNA, LNC1845 , was functionally characterized. Comprehensive bioinformatic analysis of these lncRNAs indicates that divergent lncRNAs occupied the majority of female and male germ cells. Integrating lncRNA expression into the bioinformatic analysis also enhances the cell-type classification of female germ cells. Functional dissection using in vitro differentiation of human pluripotent stem cells to germ cells revealed the regulatory role of LNC1845 on a transcription factor essential for ovarian follicle development, LHX8 , by modulating the levels of histone modifications, H3K4me3 and H3K27Ac. Hence, bioinformatical analysis and experimental verification provide a comprehensive analysis of lncRNAs in developing germ cells and elucidate how an lncRNA function as a cis regulator during human germ cell development.
Keyphrases
- induced apoptosis
- transcription factor
- cell cycle arrest
- single cell
- endothelial cells
- pluripotent stem cells
- cell death
- endoplasmic reticulum stress
- machine learning
- stem cells
- oxidative stress
- poor prognosis
- genome wide identification
- induced pluripotent stem cells
- rna seq
- long noncoding rna
- high throughput
- deep learning
- pi k akt
- single molecule
- binding protein