Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation.
Elize WolmaransJuanita MelletChrisna DurandtFourie JoubertMichael Sean PepperPublished in: Stem cells international (2021)
The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs that is not well understood is their heterogeneity. The aim of this exploratory study was to characterize the heterogeneity of freshly isolated hASCs after two population doublings (P2) using single-cell transcriptome analysis. A minimum of two subpopulations were identified at P2. A major subpopulation was identified as contractile cells which, based on gene expression patterns, are likely to be pericytes and/or vascular smooth muscle cells (vSMCs).
Keyphrases
- single cell
- rna seq
- vascular smooth muscle cells
- induced apoptosis
- gene expression
- endothelial cells
- clinical trial
- cell cycle arrest
- high throughput
- skeletal muscle
- angiotensin ii
- induced pluripotent stem cells
- dna methylation
- signaling pathway
- pluripotent stem cells
- cell death
- oxidative stress
- genome wide
- smooth muscle
- randomized controlled trial
- cell proliferation
- climate change
- phase ii