A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles.
Raquel Fernández-PenasCristobal Verdugo-EscamillaCarla TriunfoStefanie GärtnerAnnarita D'UrsoFrancesca OltolinaAntonia FollenziGabriele MaoloniHelmut CölfenGiuseppe FaliniJaime Gómez MoralesPublished in: Journal of materials chemistry. B (2023)
We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species Crassostrea gigas (Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H 3 PO 4 , KH 2 PO 4 , and K 2 HPO 4 ), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25-200 °C), and digestion times (1 week-2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using K 2 HPO 4 instead of KH 2 PO 4 at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place via a dissolution-reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering.
Keyphrases
- tissue engineering
- mesenchymal stem cells
- sewage sludge
- induced apoptosis
- anaerobic digestion
- heavy metals
- municipal solid waste
- bone regeneration
- risk assessment
- cell cycle arrest
- nlrp inflammasome
- visible light
- bone marrow
- lactic acid
- soft tissue
- oxidative stress
- cell therapy
- cell death
- mass spectrometry
- life cycle
- postmenopausal women
- pluripotent stem cells
- placebo controlled
- atomic force microscopy
- pi k akt
- study protocol
- high speed