OsPP2C09 Is a Bifunctional Regulator in Both ABA-Dependent and Independent Abiotic Stress Signaling Pathways.
Myung Ki MinRigyeong KimWoo-Jong HongKi-Hong JungJong-Yeol LeeBeom-Gi KimPublished in: International journal of molecular sciences (2021)
Clade A Type 2C protein phosphatases (PP2CAs) negatively regulate abscisic acid (ABA) signaling and have diverse functions in plant development and in response to various stresses. In this study, we showed that overexpression of the rice ABA receptor OsPYL/RCAR3 reduces the growth retardation observed in plants exposed to osmotic stress. By contrast, overexpression of the OsPYL/RCAR3-interacting protein OsPP2C09 rendered plant growth more sensitive to osmotic stress. We tested whether OsPP2CAs activate an ABA-independent signaling cascade by transfecting rice protoplasts with luciferase reporters containing the drought-responsive element (DRE) or ABA-responsive element (ABRE). We observed that OsPP2CAs activated gene expression via the cis-acting drought-responsive element. In agreement with this observation, transcriptome analysis of plants overexpressing OsPP2C09 indicated that OsPP2C09 induces the expression of genes whose promoters contain DREs. Further analysis showed that OsPP2C09 interacts with DRE-binding (DREB) transcription factors and activates reporters containing DRE. We conclude that, through activating DRE-containing promoters, OsPP2C09 positively regulates the drought response regulon and activates an ABA-independent signaling pathway.
Keyphrases
- transcription factor
- arabidopsis thaliana
- plant growth
- signaling pathway
- gene expression
- genome wide identification
- crispr cas
- binding protein
- genome editing
- dna binding
- cancer therapy
- pi k akt
- heat stress
- climate change
- dna methylation
- poor prognosis
- genome wide
- protein protein
- drug delivery
- rna seq
- small molecule
- genome wide analysis