Genome-wide off-target analyses of CRISPR/Cas9-mediated T-cell receptor engineering in primary human T cells.

Theresa KaeuferleTanja A StiefStefan CanzarNayad N KutluSemjon WillierDana StengerPaulina Ferrada-ErnstNicola HabjanAnnika E PetersDirk H BuschTobias Feuchtinger

Published in: Clinical & translational immunology (2022)

The combinatorial approach of whole-genome sequencing and targeted deep sequencing confirmed highly specific genetic engineering using CRISPR/Cas9-mediated TCR knockout without potentially harmful exonic off-target effects.

Keyphrases

crispr cas
genome wide
genome editing
endothelial cells
dna methylation
copy number
regulatory t cells
single cell
induced pluripotent stem cells
pluripotent stem cells
immune response
drug delivery