A Limonoid Kihadanin B from Immature Citrus unshiu Peels Suppresses Adipogenesis through Repression of the Akt-FOXO1-PPARγ Axis in Adipocytes.
Shizuka BabaYasuaki UenoTakashi KikuchiReiko TanakaKo FujimoriPublished in: Journal of agricultural and food chemistry (2016)
Citrus limonoids are secondary metabolites and exhibit a variety of biological activities. In this study, we elucidated the suppression of adipogenesis by a Citrus limonoid kihadanin B and determined its molecular mechanism in mouse 3T3-L1 adipocytes. Kihadanin B was purified from the peels of immature Citrus unshiu by HPLC, and its chemical structure was determined by NMR and mass spectrometry. Kihadanin B reduced the lipid accumulation with the reduction of the expression levels of the adipogenic and lipogenic genes, but did not affect lipolysis in adipocytes. Phosphorylation levels of Akt and a forkhead transcriptional factor, FOXO1, a repressor of PPARγ, were lowered by kihadanin B. Furthermore, kihadanin B increased the binding level of FOXO1 to the PPARγ gene promoter in adipocytes. These results indicate that a Citrus limonoid kihadanin B repressed the adipogenesis by decreasing lipid accumulation through the suppression of the Akt-FOXO1-PPARγ axis in 3T3-L1 adipocytes.
Keyphrases
- signaling pathway
- transcription factor
- adipose tissue
- high fat diet induced
- insulin resistance
- pi k akt
- mass spectrometry
- genome wide identification
- cell proliferation
- ms ms
- dna binding
- genome wide
- high resolution
- poor prognosis
- magnetic resonance
- metabolic syndrome
- fatty acid
- dna methylation
- liquid chromatography
- type diabetes
- skeletal muscle
- binding protein
- tandem mass spectrometry
- oxidative stress
- protein kinase
- capillary electrophoresis
- long non coding rna
- heat stress