Mass spectrometry imaging protocol for spatial mapping of lipids, N-glycans and peptides in murine lung tissue.
Yuen T NgaiMatthew T BriggsPeter HoffmannClifford YoungEmma Parkinson-LawrenceManuela Klingler-HoffmannSandra OrgeigPeter HoffmannPublished in: Rapid communications in mass spectrometry : RCM (2024)
Our protocol provides instructions on sample preparation for high spatial resolution MALDI-MSI, MS/MS data acquisition and lipid, N-glycan and peptide annotation and identification from murine lungs. This protocol will allow non-biased analyses of diseased lungs from preclinical murine models and provide further insight into disease models.