Alpha-Lipoic Acid Promotes Intestinal Epithelial Injury Repair by Regulating MAPK Signaling Pathways.
Yu YangYong XiaoYue JiangJiajun LuoJingwen YuanJunfeng YanQiang TongPublished in: Mediators of inflammation (2022)
Intestinal epithelial cells are an essential barrier in human gastrointestinal tract, and healing of epithelial wound is a key process in many intestinal diseases. α -Lipoic acid (ALA) was shown to have antioxidative and anti-inflammatory effects, which could be helpful in intestinal epithelial injury repair. The effects of ALA in human colonic epithelial cells NCM460 and human colorectal adenocarcinoma cells Caco-2 were studied. ALA significantly promoted NCM460 and Caco-2 migration, increased mucosal tight junction factors ZO-1 and OCLN expression, and ALA accelerated cell injury repair of both cells in wound healing assay. Western blot analysis indicated that ALA inhibited a variety of mitogen-activated protein kinase (MAPK) signaling pathways in the epithelial cells. In conclusion, ALA was beneficial to repair of intestinal epithelial injury by regulating MAPK signaling pathways.
Keyphrases
- signaling pathway
- induced apoptosis
- pi k akt
- endothelial cells
- cell cycle arrest
- oxidative stress
- induced pluripotent stem cells
- wound healing
- pluripotent stem cells
- poor prognosis
- epithelial mesenchymal transition
- squamous cell carcinoma
- stem cells
- radiation therapy
- cell proliferation
- blood brain barrier
- south africa
- bone marrow
- mesenchymal stem cells
- tyrosine kinase
- ulcerative colitis
- protein kinase
- solid state