The polysaccharide AMP as one main bioactive component of Astragalus membranaceus (Fisch.) Bunge was separated and characterized. The results showed that AMP was a typical acidic heteropolysaccharide dominated by glucose, galacturonic acid and arabinose with typical shear-thinning and fluid-like behavior. Scanning electron microscopy images showed that AMP existed in the state of lamellar aggregates with a smooth compact surface. In addition, AMP exhibited strong antioxidant activity with an oxygen radical absorption capacity value of 278.68 ± 9.31 μM TE per g, and excellent α-glucosidase inhibitory activity and cholate binding ability. Furthermore, in vivo , AMP treatment significantly decreased blood glucose and serum insulin levels, improved glucose intolerance and insulin resistance, regulated the blood lipid profile, alleviated oxidative stress, and relieved liver damage in type 2 diabetes mellitus (T2DM) mice. Pearson correlation analysis suggested that the mitigation of oxidative stress contributed to the hypoglycemic effect of AMP, indicating that it is a beneficial functional food ingredient for T2DM.
Keyphrases
- protein kinase
- blood glucose
- oxidative stress
- glycemic control
- water soluble
- electron microscopy
- insulin resistance
- type diabetes
- dna damage
- adipose tissue
- deep learning
- blood pressure
- transcription factor
- climate change
- ischemia reperfusion injury
- diabetic rats
- high fat diet induced
- metabolic syndrome
- machine learning
- weight loss
- ionic liquid
- molecular docking
- skeletal muscle
- replacement therapy
- human health
- dna binding
- data analysis
- binding protein
- heat stress