Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi.
Tim M van LeeuweMark ArentshorstTim ErnstEbru AlaziPeter J PuntArthur F J RamPublished in: Fungal biology and biotechnology (2019)
Our findings advocate the use of CRISPR/Cas9 to create multiple gene deletions in both a fast and reliable way, while simultaneously omitting possible locus-dependent-side-effects of poor auxotrophic marker expression.