Protective Effect of Lycium ruthenicum Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats.
Hua GaoYanzhong XueLingyu WuJinghong HuoYufei PangJingxin ChenQinghan GaoPublished in: Molecules (Basel, Switzerland) (2022)
Acrylamide (ACR) is formed during tobacco and carbohydrate-rich food heating and is widely applied in many industries, with a range of toxic effects. The antioxidant properties of Lycium ruthenicum polyphenols (LRP) have been established before. This study aimed to research the protective effect of LRP against ACR-induced liver injury in SD rats. Rats were divided into six groups: Control, ACR (40 mg/kg/day, i.g.), LRP (50, 100, and 200 mg/kg/day, i.g.) plus ACR, and LRP groups. After 19 days, we evaluated oxidative status and mitochondrial functions in the rat's liver. The results showed that glutathione (GSH) and superoxide dismutase (SOD) levels increased after LRP pretreatment. In contrast, each intervention group reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels compared to the ACR group. Meanwhile, alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver mitochondrial ATPase activity, mRNA expression of mitochondrial complex I, III, and expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its downstream proteins were all increased. This study suggested that LRP could reduce ACR-induced liver injury through potent antioxidant activity. LRP is recommended as oxidative stress reliever against hepatotoxicity.
Keyphrases
- oxidative stress
- diabetic rats
- reactive oxygen species
- dna damage
- nuclear factor
- low density lipoprotein
- ischemia reperfusion injury
- induced apoptosis
- randomized controlled trial
- toll like receptor
- magnetic resonance
- magnetic resonance imaging
- nitric oxide
- climate change
- heat shock
- cell proliferation
- breast cancer cells
- endoplasmic reticulum